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| Wed 10-12 | RFU ACE Girls Stage 1 2025-26 | Worthing College Girls U18 | Hartpury College Girls U18 (Ace) | Ref: Joseph Garman |
| Sat 13-12 | Regional 1 South Central 2025-26 | Camberley 1 | Worthing | Ref: Matthew Holmes |
| Sat 13-12 | Regional 1 South Central 2025-26 | CS Rugby 1863 1 | Old Alleynians 1 | Ref: Ben Stewart |
| Sat 13-12 | Regional 1 South Central 2025-26 | Hammersmith & Fulham 1 | Maidenhead | Ref: Matt Blackett |
| Sat 13-12 | Regional 1 South Central 2025-26 | Wimbledon 1 | Tunbridge Wells | Ref: Chris Scott |
| Sat 13-12 | Regional 1 South East 2025-26 | Amersham & Chiltern | Old Northamptonians 1st | Ref: Ollie Carter |
| Sat 13-12 | Regional 1 South East 2025-26 | Letchworth | North Walsham | Ref: Josh Bamber |
| Sat 13-12 | Regional 1 South East 2025-26 | Medway | Colchester 1 | Ref: Rob Hitch |
| Sat 13-12 | Regional 1 South East 2025-26 | Sudbury 1 | Tring | Ref: Josh Methven |
| Sat 13-12 | Regional 1 South East 2025-26 | Westcliff 1 | Hertford | Ref: Jesse Lipetz-robic |
| Sat 20-12 | Regional 1 South Central 2025-26 | Farnham 1 | Camberley 1 | Ref: Chris Scott |
| Sat 20-12 | Regional 1 South Central 2025-26 | London Scottish Lions 1st XV | Wimbledon 1 | Ref: Jesse Lipetz-robic |
| Sat 20-12 | Regional 1 South Central 2025-26 | Old Alleynians 1 | Hammersmith & Fulham 1 | |
| Sat 20-12 | Regional 1 South Central 2025-26 | Tunbridge Wells | CS Rugby 1863 1 | Ref: Josh Bamber |
| Sat 20-12 | Regional 1 South Central 2025-26 | Worthing | Jersey RFC | Ref: Rob Hitch |
| Sat 20-12 | Regional 1 South East 2025-26 | Colchester 1 | Westcliff 1 | Ref: Ben Stewart |
| Sat 20-12 | Regional 1 South East 2025-26 | Hertford | Letchworth | Ref: Josh Methven |
| Sat 20-12 | Regional 1 South East 2025-26 | North Walsham | Sudbury 1 | Ref: Matthew Holmes |
| Sat 20-12 | Regional 1 South East 2025-26 | Southend 1 | Medway | Ref: Ollie Carter |
| Sat 20-12 | Regional 1 South East 2025-26 | Tring | Amersham & Chiltern | Ref: Matt Blackett |