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| Sat 14-03 | Regional 1 South East 2025-26 | North Walsham | Southend 1 | Ref: Ben Stewart |
| Sat 21-03 | Regional 1 South Central 2025-26 | Farnham 1 | Old Alleynians 1 | Ref: Sam Peters |
| Sat 21-03 | Regional 1 South Central 2025-26 | Jersey RFC | Camberley 1 | Ref: Chris Scott |
| Sat 21-03 | Regional 1 South Central 2025-26 | London Scottish Lions 1st XV | Hammersmith & Fulham 1 | Ref: Matthew Holmes |
| Sat 21-03 | Regional 1 South Central 2025-26 | Wimbledon 1 | Bracknell | Ref: James MABY |
| Sat 21-03 | Regional 1 South Central 2025-26 | Worthing | CS Rugby 1863 1 | Ref: Rob Hitch |
| Sat 21-03 | Regional 1 South East 2025-26 | Colchester 1 | Sudbury 1 | |
| Sat 21-03 | Regional 1 South East 2025-26 | Medway | Shelford 1 | Ref: Ben Stewart |
| Sat 21-03 | Regional 1 South East 2025-26 | Southend 1 | Letchworth | Ref: Ollie Carter |
| Sat 21-03 | Regional 1 South East 2025-26 | Tring | Hertford | Ref: Max Marshall |
| Sat 21-03 | Regional 1 South East 2025-26 | Westcliff 1 | Amersham & Chiltern | Ref: William Foster |
| Sat 28-03 | Regional 1 South Central 2025-26 | Camberley 1 | Wimbledon 1 | Ref: William Foster |
| Sat 28-03 | Regional 1 South Central 2025-26 | CS Rugby 1863 1 | Farnham 1 | |
| Sat 28-03 | Regional 1 South Central 2025-26 | Hammersmith & Fulham 1 | Worthing | |
| Sat 28-03 | Regional 1 South Central 2025-26 | Old Alleynians 1 | Maidenhead | |
| Sat 28-03 | Regional 1 South Central 2025-26 | Tunbridge Wells | Jersey RFC | Ref: Josh Bamber |
| Sat 28-03 | Regional 1 South East 2025-26 | Amersham & Chiltern | Colchester 1 | Ref: Max Marshall |
| Sat 28-03 | Regional 1 South East 2025-26 | Hertford | Medway | Ref: Chris Scott |
| Sat 28-03 | Regional 1 South East 2025-26 | Letchworth | Old Northamptonians 1st | |
| Sat 28-03 | Regional 1 South East 2025-26 | North Walsham | Tring | Ref: Jesse Lipetz-robic |
| Sat 28-03 | Regional 1 South East 2025-26 | Sudbury 1 | Southend 1 | Ref: Matthew Holmes |
| Sat 11-04 | Regional 1 South Central 2025-26 | Farnham 1 | Hammersmith & Fulham 1 | |
| Sat 11-04 | Regional 1 South Central 2025-26 | London Scottish Lions 1st XV | Camberley 1 | |
| Sat 11-04 | Regional 1 South Central 2025-26 | Tunbridge Wells | Old Alleynians 1 | |
| Sat 11-04 | Regional 1 South Central 2025-26 | Worthing | Bracknell | |
| Sat 11-04 | Regional 1 South East 2025-26 | Colchester 1 | Shelford 1 | |
| Sat 11-04 | Regional 1 South East 2025-26 | Hertford | North Walsham | |
| Sat 11-04 | Regional 1 South East 2025-26 | Southend 1 | Amersham & Chiltern | |
| Sat 11-04 | Regional 1 South East 2025-26 | Tring | Letchworth | |
| Sat 11-04 | Regional 1 South East 2025-26 | Westcliff 1 | Medway |