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| Wed 28-10 | RFU ACE Boys Tier 2 South Pool 2026-27 | Duke Of York's Royal Military School U18 | City of Oxford College | |
| Sat 31-10 | Regional 1 North West 2026-27 | Macclesfield 1XV | Rochdale 1XV | Ref: Ben Stewart |
| Sat 31-10 | Regional 1 South Central 2026-27 | CS Rugby 1863 1 | Worthing | Ref: Oliver King |
| Sat 31-10 | Regional 1 South Central 2026-27 | Farnham 1 | Tunbridge Wells | Ref: James MABY |
| Sat 31-10 | Regional 1 South Central 2026-27 | Sevenoaks | London Scottish Lions 1st XV | Ref: Josh Methven |
| Sat 31-10 | Regional 1 South East 2026-27 | Hertford | Southend 1 | Ref: Sam Peters |
| Sat 31-10 | Regional 1 South East 2026-27 | North Walsham | Northampton Old Scouts 1st | Ref: Doug Hampshire |
| Sat 31-10 | Regional 1 South East 2026-27 | Old Colfeians 1 | Eton Manor 1 | Ref: Jack Trevella |
| Sat 31-10 | Regional 1 South East 2026-27 | Sidcup | Old Northamptonians 1st | Ref: Matt Blackett |
| Sat 31-10 | Regional 1 South East 2026-27 | Sudbury 1 | Tring | Ref: Chris Scott |
| Sat 31-10 | Regional 1 South East 2026-27 | Westcliff 1 | Letchworth | Ref: Rob Hitch |
| Sat 14-11 | Regional 1 South Central 2026-27 | CS Rugby 1863 1 | Sevenoaks | |
| Sat 14-11 | Regional 1 South Central 2026-27 | Old Alleynians 1 | Farnham 1 | |
| Sat 14-11 | Regional 1 South Central 2026-27 | Tunbridge Wells | London Scottish Lions 1st XV | |
| Sat 14-11 | Regional 1 South Central 2026-27 | Wimbledon 1 | Bracknell | |
| Sat 14-11 | Regional 1 South Central 2026-27 | Worthing | Bournemouth | |
| Sat 14-11 | Regional 1 South East 2026-27 | Eton Manor 1 | Sidcup | |
| Sat 14-11 | Regional 1 South East 2026-27 | Letchworth | North Walsham | |
| Sat 14-11 | Regional 1 South East 2026-27 | Tring | Southend 1 | |
| Sat 14-11 | Regional 1 South East 2026-27 | Westcliff 1 | Hertford | |
| Sat 21-11 | Regional 1 Midlands 2026-27 | Banbury | Moseley Oak 1 | |
| Sat 21-11 | Regional 1 South Central 2026-27 | Farnham 1 | Brunel University Mens 1s | |
| Sat 21-11 | Regional 1 South Central 2026-27 | London Scottish Lions 1st XV | Old Alleynians 1 | |
| Sat 21-11 | Regional 1 South Central 2026-27 | Sevenoaks | Tunbridge Wells | |
| Sat 21-11 | Regional 1 South East 2026-27 | Hertford | Tring | Ref: Callum Folan |
| Sat 21-11 | Regional 1 South East 2026-27 | North Walsham | Westcliff 1 | |
| Sat 21-11 | Regional 1 South East 2026-27 | Old Colfeians 1 | Letchworth | |
| Sat 21-11 | Regional 1 South East 2026-27 | Sidcup | Northampton Old Scouts 1st | |
| Sat 21-11 | Regional 1 South East 2026-27 | Southend 1 | Old Northamptonians 1st | |
| Sat 21-11 | Regional 1 South East 2026-27 | Sudbury 1 | Eton Manor 1 |