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| Sat 14-03 | Regional 1 Tribute Ale South West 2025-26 | Launceston | St Austell | Ref: Spencer Pearce |
| Sat 21-03 | Regional 1 Midlands 2025-26 | Banbury | Bournville 1 | Ref: Oli Carey |
| Sat 21-03 | Regional 1 South Central 2025-26 | Farnham 1 | Old Alleynians 1 | Ref: Sam Peters |
| Sat 21-03 | Regional 1 South Central 2025-26 | Jersey RFC | Camberley 1 | Ref: Chris Scott |
| Sat 21-03 | Regional 1 South Central 2025-26 | Maidenhead | Tunbridge Wells | Ref: Scott de Weymarn |
| Sat 21-03 | Regional 1 Tribute Ale South West 2025-26 | Launceston | Devonport Services | Ref: George Berry |
| Sat 21-03 | Regional 1 Tribute Ale South West 2025-26 | Marlborough | Lydney | Ref: ASHLEY MORRIS |
| Sat 21-03 | Regional 1 Tribute Ale South West 2025-26 | Royal Wootton Bassett | Brixham | Ref: Spencer Pearce |
| Sat 21-03 | Regional 1 Tribute Ale South West 2025-26 | Sidmouth | Chew Valley | Ref: Ollie Parsons |
| Sat 21-03 | Regional 1 Tribute Ale South West 2025-26 | St Austell | Matson | Ref: Connor Robinson |
| Sat 21-03 | Regional 1 Tribute Ale South West 2025-26 | Topsham | Exmouth | Ref: Alex Mulcahy |
| Sat 28-03 | Regional 1 Midlands 2025-26 | Drybrook | Banbury | Ref: George Berry |
| Sat 28-03 | Regional 1 Midlands 2025-26 | Newent | Lichfield | Ref: Jamie Hanson |
| Sat 28-03 | Regional 1 South Central 2025-26 | Bracknell | London Scottish Lions 1st XV | Ref: Craig Johnson |
| Sat 28-03 | Regional 1 South East 2025-26 | Amersham & Chiltern | Colchester 1 | Ref: Max Marshall |
| Sat 28-03 | Regional 1 Tribute Ale South West 2025-26 | Brixham | St Austell | Ref: Chris Hoadley |
| Sat 28-03 | Regional 1 Tribute Ale South West 2025-26 | Chew Valley | Royal Wootton Bassett | Ref: Scott de Weymarn |
| Sat 28-03 | Regional 1 Tribute Ale South West 2025-26 | Devonport Services | Marlborough | Ref: Will Williams |
| Sat 28-03 | Regional 1 Tribute Ale South West 2025-26 | Exmouth | Launceston | Ref: Ollie Parsons |
| Sat 28-03 | Regional 1 Tribute Ale South West 2025-26 | Lydney | Sidmouth | Ref: Alex Mulcahy |
| Sat 28-03 | Regional 1 Tribute Ale South West 2025-26 | Topsham | Matson | Ref: Alex Peggie |
| Sat 11-04 | Regional 1 Midlands 2025-26 | Banbury | Bromsgrove 1 | Ref: Alex Mulcahy |
| Sat 11-04 | Regional 1 South Central 2025-26 | Jersey RFC | Wimbledon 1 | Ref: Oli Carey |
| Sat 11-04 | Regional 1 South Central 2025-26 | Maidenhead | CS Rugby 1863 1 | Ref: Craig Johnson |
| Sat 11-04 | Regional 1 Tribute Ale South West 2025-26 | Marlborough | Exmouth | Ref: Chris Hoadley |
| Sat 11-04 | Regional 1 Tribute Ale South West 2025-26 | Matson | Brixham | Ref: ASHLEY MORRIS |
| Sat 11-04 | Regional 1 Tribute Ale South West 2025-26 | Royal Wootton Bassett | Lydney | Ref: Callum Folan |
| Sat 11-04 | Regional 1 Tribute Ale South West 2025-26 | Sidmouth | Devonport Services | Ref: Spencer Pearce |
| Sat 11-04 | Regional 1 Tribute Ale South West 2025-26 | St Austell | Chew Valley | Ref: George Berry |
| Sat 11-04 | Regional 1 Tribute Ale South West 2025-26 | Topsham | Launceston | Ref: Scott de Weymarn |